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. 2014 Dec 4;10(12):e1004825. doi: 10.1371/journal.pgen.1004825

Figure 6. RANBP9 binds numerous mRNAs and affects their expression levels at least partially through affecting alternative splicing.

Figure 6

(A) A representative mRNA assembly output showing RIP-Seq reads for Ddx25 identified from the RIP products using the RANBP9 antibody and IgG (control). (B) qPCR analyses of levels of three RANBP9-bound mRNAs (Rfx2, Plec and Usp19) in WT and gcKO testes. All three are highly enriched in WT compared to gcKO testes, demonstrating the specificity of the anti-RANBP9 antibody used in RIP-Seq assays. (C) GO enrichment analyses of RANBP9-bound mRNAs identified using RIP-Seq. (D) Venn diagram showing the number of up- and down-regulated RANBP9-bound target transcripts in gcKO testes (P<0.05, fold change>1.5). (E) qPCR analyses of levels of 17 RANBP9 target transcripts in 6-week-old WT and gcKO testes. Data are presented as mean ± SEM, and significantly altered levels were marked with * (n = 4, P<0.05).