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. 2014 Dec 4;10(12):e1004535. doi: 10.1371/journal.ppat.1004535

Figure 6. Viral exocytosis does not occur at specialized sites depleted in cytoskeleton proteins.

Figure 6

(A) Cells were transduced to express mCherry-actin. Long actin stress fibers are indicated (arrows). (B) Cells were transduced to express mCherry-actin, infected with PRV 486 expressing gM-pHluorin, and imaged at 4.5–5 hr after PRV infection. The image is a maximum difference projection depicting virus particle exocytosis events (green circles) over a 10.5 min time course. (C) Still images depicting a single exocytosis event in panel B. (D) Ensemble averages of gM-pHluorin (top, green line) and mCherry-actin (bottom, red line) relative fluorescence. Shaded area represents standard deviation. Data represent 34 exocytosis events in 3 independent experiments. (E) Cells were transduced to express mCherry-tubulin and infected with PRV as above. The image is a maximum difference projection depicting virus particle exocytosis events (green circles) over a 9 min time course. (F) Still images depicting a single exocytosis event in panel E. (G) Ensemble averages of gM-pHluorin (top, green line) and mCherry-tubulin (bottom, red line) relative fluorescence. Shaded area represents standard deviation. Data represent 24 exocytosis events in 3 independent experiments. Scale bars represent 2 µm in all images.