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. 2014 Dec 4;10(12):e1004827. doi: 10.1371/journal.pgen.1004827

Table 2. c-Kit POL II traveling ratio.

IkWT IkHT IkNULL DMSO Fvp
+5/−0.5 2.1 0.9 1 1.9 0.8
+9.7/−0.5 1.4 0.5 0.3 1.6 0.3

c-Kit traveling ratio values (as defined by the relative ratio of POL II density in gene ORF vs. promoter-proximal regions) were obtained by chromatin immunoprecipitation with POL II antibody, which recognizes the N-terminal region of the large subunit of POL II and binds POL II in a phosphorylation-independent manner and indicate the enrichment levels of c-Kit +5/−0.5 or +9,7/−0.5 regions relative to the control and the input samples (see also Figure 2 and 3 legends); IkWT: Ikaros wild type HPCs; IkHT: Ikaros heterozygote null HPCs; IkNULL: Ikaros homozygote null lin HPCs; DMSO: Dimethyl sulfoxide-treated G1E2 cells (0.01% for 2 h); Fvp: Flavopiridol-treated G1E2 cells (100 nM for 2 h).