Table 1. Primer list for cloning.
Primer name | Sequence (5′ to 3′) |
LucNcoIF | GTACCATGGAAGACGCCAAAAACATAAAG |
LucPstIR | ATACAGCTGCAGCCTACAATTTGGACTTTC |
pFlgBF | ACTGCTAGCAATAGAATTCATAATTGGAAT |
pFlgBR | CACCCATGGTTTCTCCCCCTTCTCAAAA |
Boldface indicates restriction sites.