Table 1. Sequences of C. psittaci and adenovirus primers and probes used in the study.

Pathogen	Type of PCR	Target gene	Target length (base pairs)	Primer/probe sequences
Chlamydophila psittaci	Real-time qPCR	ITS	145	Forward 5′-TTGGTCTGTAAATTATTGATCC-3′
				Reverse 5′-CATTTAGTTTACGATCAAGTATG-3′
				Probe 5′-FAM-ATGCAAGTTAACWTCACCTAAAGACAT-BHQ1-3′
Adenovirus*	Conventional PCR	pol	324	Forward 5′-GTCTACGAYATHTGYGGMATGTA-3′
				Reverse 5′-TCTCCATCCTCDRTTRTGVA-3′
Psittacine adenovirus HKU1	Conventional PCR	pol	175	Forward 5′-GGGTGACTATGTCTATCGACG-3′
				Reverse 5′-GATTTCATACTTCGACAGCG-3′
Psittacine adenovirus HKU1	Real-time qPCR	pol	175	Forward 5′-GGGTGACTATGTCTATCGACG-3′
				Reverse 5′-GATTTCATACTTCGACAGCG-3′
				Probe 5′-FAM-AGGTAGTGTGTCGAGGCGCT-BHQ1-3′

qPCR, quantitative polymerase chain reaction.

*Consensus primers for adenovirus were designed by performing multiple alignments of pol genes of adenovirus and related sequences available in NCBI GenBank.