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. 2014 Dec 4;10(12):e1004847. doi: 10.1371/journal.pgen.1004847

Figure 2. Condensins I and II are both required for proper development of the cerebral cortex.

Figure 2

(A) Genotypes of mice used in the current study. The heterozygotes of Ncaph or Ncaph2 mutants in the NesCre background were used as a control (Ctrl). (B) Frozen sections of embryonic brains from cKO mice at E13.5 or E16.5 were immunolabeled with cell-type specific markers: SOX2 for NSCs; TBR1 for deep-layer neurons; BRN2 for upper-layer neurons. DNA was counterstained with Hoechst in the upper and middle panels. The data shown are from a single representative experiment out of three repeats. Sections from at least two different embryos of each genotype were analyzed. Bar, 100 µm. (C) The numbers of SOX2-positive cells were measured in the dorsal area of cortices in control and cKO mice. Data were obtained from four independent sections from two different embryos and normalized to the mean number of NSCs in control mice as 100%. Bars indicate the mean and SD. * P<0.05, *** P<0.001 (t-test with a Holm correction for multiple comparisons). (D) The numbers of cells present in the CP were measured. Data were obtained and analyzed as in (C). (E) The ratios of TBR1-positive, BRN2-positive, and double-positive (DP) cells were measured in the CP at E16.5. Data were obtained from two different embryos (at least 600 neurons scored). *** P<0.001 (Chi-squared test).