Table 5. Descriptive statistics and stability values of candidate reference genes under light/dark cycling, all-dark growth, and temperature fluctuations (n = 30).
btl | tbp | vma2 | vma1 | adk | vma3 | acl | asl | act | l6 | |
geo Mean [CT] | 24.68 | 24.80 | 25.54 | 26.23 | 25.39 | 24.01 | 26.16 | 23.35 | 21.08 | 21.85 |
ar Mean [CT] | 24.70 | 24.83 | 25.57 | 26.26 | 25.42 | 24.05 | 26.19 | 23.40 | 21.13 | 21.91 |
min [CT] | 22.16 | 22.94 | 23.21 | 24.27 | 22.84 | 21.62 | 23.73 | 21.37 | 18.60 | 19.04 |
max [CT] | 26.76 | 27.86 | 27.63 | 28.59 | 27.78 | 26.55 | 28.79 | 26.33 | 24.29 | 25.14 |
SD [±CT] | 0.750 | 0.927 | 0.956 | 0.982 | 1.088 | 1.137 | 1.193 | 1.257 | 1.341 | 1.415 |
CV [% CT] | 3.036 | 3.733 | 3.739 | 3.741 | 4.280 | 4.729 | 4.555 | 5.370 | 6.344 | 6.459 |
r | 0.810 | 0.903 | 0.880 | 0.973 | 0.892 | 0.951 | 0.961 | 0.939 | 0.955 | 0.962 |
p | 0.001 | 0.001 | 0.001 | 0.001 | 0.001 | 0.001 | 0.001 | 0.001 | 0.001 | 0.001 |
NF Stability value | 0.191 | 0.137 | 0.178 | 0.129 | 0.230 | 0.243 | 0.279 | 0.148 | 0.185 | 0.218 |
Int Var: Dark Flux | 0.129 | 0.003 | 0.007 | 0.004 | 0.022 | 0.019 | 0.129 | 0.023 | 0.040 | 0.025 |
Int Var: Light Flux | 0.089 | 0.136 | 0.001 | 0.001 | 0.150 | 0.001 | 0.166 | 0.002 | 0.025 | 0.300 |
Int Var: All Dark | 0.040 | 0.024 | 0.020 | 0.032 | 0.254 | 0.058 | 0.051 | 0.156 | 0.066 | 0.067 |
Int Var: 25°C | 0.074 | 0.006 | 0.115 | 0.000 | 0.012 | 0.011 | 0.066 | 0.022 | 0.015 | 0.153 |
Int Var: 37°C | 0.041 | 0.093 | 0.017 | 0.026 | 0.266 | 0.003 | 0.271 | 0.006 | 0.018 | 0.050 |
NF = NormFinder, Int Var = Intragroup Variation as calculated by Normfinder.