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. 2014 Oct 9;289(49):33741–33753. doi: 10.1074/jbc.M114.582353

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FIGURE 3. — LYPLA2 siRNA knockdown in PC3, MDA-MB-231, and LNCaP cells. A, PGE₂-G hydrolytic activity of cytosol obtained from PC3, MDA-MB-231, and LNCaP control (scrambled) or LYPLA2-deficient (siRNA) cells. Western blot analysis of LYPLA2 in control (scrambled) and LYPLA2-depleted (siRNA) PC3 (B), MDA-MB-231 (C and D), or LNCaP (E) cells. β-Actin Western blotting verified equaling protein loading (5 μg per lane). Data are presented as the mean ± S.D. of triplicate analyses. ****, p < 0.001; **, p < 0.01.