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. 2014 Oct 9;289(49):33741–33753. doi: 10.1074/jbc.M114.582353

FIGURE 5.

FIGURE 5.

LYPLA1 siRNA knockdown in MDA-MB-231 cells and LYPLA1 overexpression in HEK293. A, Western blot analysis of LYPLA1 in control MDA-MB-231 cells (scrambled) and LYPLA1-depleted cells (siRNA). β-Actin Western blotting verified uniform protein loading (5 μg per lane). B, PGE2-G hydrolytic activity of cytosol obtained from MDA-MB-231 control cells (scrambled) or LYPLA1-deficient MDA-MB-231 cells (siRNA). C, Western blot analysis of LYPLA1 in control HEK293 cells (control) and LYPLA2-overexpressing HEK293 cells (cDNA). β-Actin Western blotting verified equaling protein loading (5 μg per lane). D, PGE2-G hydrolytic activity of cytosol obtained from HEK293 (control) or LYPLA1-overexpressing (cDNA) cells. Data are presented as the mean ± S.D. of triplicate analyses. **, p < 0.01 by t test. N/S indicates no significance.