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. 2014 Oct 9;289(49):33741–33753. doi: 10.1074/jbc.M114.582353

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Production and hydrolytic activity of recombinant human LYPLA1 and LYPLA2 by overexpression in E. coli. A, PGE₂-G hydrolytic activity of cytosol obtained from wild-type E. coli and E. coli overexpressing recombinant human LYPLA1 and recombinant human LYPLA2. B, Coomassie Blue staining of recombinant LYPLA2 (left) and recombinant LYPLA1 (right) by E. coli compared with control. C, PGE₂-G hydrolytic activity of cytosol obtained from wild-type E. coli and E. coli overexpressing recombinant human LYPLA2 or recombinant His-tagged human LYPLA2. D, Coomassie Blue staining (left) and Western blot analysis of LYPLA2 (center) and His tag (right) of purified, recombinant His-tagged LYPLA2 produced by E. coli.