TABLE 1.
Kinetic parameters for hydrolysis of multiple lipid substrates by recombinant human LYPLA2
| Substrate | kcat | Km | kcat/Km |
|---|---|---|---|
| min−1 | μm | min−1 μm−1 | |
| PGE2-G | 14.1 ± 0.4 | 13 ± 1.1 | 1.08 |
| PGF2α-G | 2.2 ± 0.3 | 5 ± 1.1 | 0.44 |
| PGD2-G | 9.5 ± 1.6 | 67 ± 14 | 0.14 |
| Lyso-PC (16:0) | 33.3 ± 1.7 | 8.3 ± 1.0 | 4.0 |
| Lyso-PC (18:0) | 44.3 ± 10.2 | 7.8 ± 4.4 | 5.6 |
| Lyso-PC (18:1) | 24.8 ± 9.1 | 40.7 ± 20.6 | 0.6 |
| Lyso-PA (16:0) | 34.6 ± 4.7 | 11.1 ± 3.2 | 3.1 |
| Lyso-PS (16:0) | 44.1 ± 10.4 | 13.3 ± 6.2 | 3.3 |
| 18:0(plasm)/20:4 plasmalogen | NAa | NA | NA |
| 1-AG | 1.7 ± 0.07 | 7.6 ± 1.2 | 0.23 |
| 2-AG | NA | NA | NA |
| AEA | NA | NA | NA |
a NA means no activity.