FIGURE 1.

Delipidation and purification of a SERCA1a-SLN complex by size exclusion chromatography. The TSK 3000SW silica gel column was pre-equilibrated and eluted in 50 mm MOPS-Tris, pH 7.5, 25 mm NaCl, 0.4 mg·ml⁻¹ DDM, supplemented with 1 mm MgCl₂ and 1 mm CaCl₂, at room temperature (20–23 °C). Conditions followed for sample preparations before injection on the column are indicated in parentheses for each panel with full description under “Experimental Procedures.” DDM or C₁₂E₈ was used at 40 mg·ml⁻¹ and NH₂OH was used at 1 m. Arrows indicate peaks corresponding to monomeric solubilized ATPase and to detergent-lipid mixed micelles. Asterisk indicates the region of the chromatogram where multimeric forms and aggregates are eluted. Excluded volume (V_o) and total volume (V_t) of the column are also indicated.