FIGURE 4.

HDAC3 affects the cellular localization of TIP60. A and B, H1299 cells were transfected with FH-TIP60, FH-TIP60 plus HDAC3, FH-TIP60 plus HDAC3-Y298F, or FH-TIP60 4KR for 24 h. An immunofluorescence assay was performed by using primary antibodies against HA and HDAC3 (see details under “Experimental Procedures”). The bar chart is representative of the percentage of cells with TIP60 cytoplasmic localization (B). C, H1299 cells were transfected with FH-TIP60 plus SIRT1 for 24 h. An immunofluorescence assay was performed using primary antibodies against FLAG and SIRT1 (see details under “Experimental Procedures”).