FIGURE 5.

HDAC3-mediated TIP60 deacetylation promotes cell survival upon DNA damage. A, HEK293 cells were transfected with p53 plus wild-type or mutant TIP60 plasmids as indicated. An immunoprecipitation assay was performed using anti-p53 antibody, and acetylated p53 Lys-120, total p53, and total TIP60 were determined by Western blot analysis. B and D, U2OS cells were transfected with the indicated plasmids or siRNA for 24 h, followed by etoposide treatment for an additional 24 h, and then stained by annexin V and PI (propidium iodide) to analyze apoptosis by FACS (see details under “Experimental Procedures”). C and E, the bar charts were plotted with the percentage of annexin V-positive cells in B and D. Data are representative of three independent experiments.