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. 2014 Oct 21;289(49):33984–33998. doi: 10.1074/jbc.M114.602581

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — NCX1 and calpain levels in isolated cardiac membrane fractions. A, NCX1 and calpain were analyzed in cytoplasmic and membrane fractions isolated from rat LV and neonatal cardiomyocytes using anti-NCX1 and anti-calpain. GAPDH and EGFR were used as controls for cytoplasmic and membrane fractions, respectively. NCX1 and calpain were analyzed in membrane fractions isolated from ABHT (B) and ABHF (C) versus sham using anti-NCX1 and anti-calpain. D, lysates from sham, ABHT, and ABHF were subjected to immunoprecipitation (IP) using anti-NCX1. The presence of endogenous NCX1 and calpain in immunoprecipitates was analyzed by immunoblotting. B–D, differences were tested using unpaired Student's t test (*, p < 0.05; **, p < 0.001) (n = 4–6). Error bars, S.E.