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. 2014 Oct 21;289(49):34033–34048. doi: 10.1074/jbc.M114.607267

FIGURE 1.

FIGURE 1.

In vitro interactions between the amino- and carboxyl-terminal regions of MyoGEF. A, schematic of MyoGEF polypeptides that were used for the GST pulldown assays in C and the immunoprecipitation assays in D. The numbers indicate the amino acid residues. B, Coomassie Blue staining image of GST-MyoGEF-501–790 used in the GST pulldown assays in C. C, GST pulldown assays. Agarose beads associated with GST-MyoGEF-501–790 were used to pull down the in vitro-translated Myc-MyoGEF-1–515. D, coimmunoprecipitation of His-MyoGEF-501–790 and the in vitro-translated Myc-DH or Myc-PH. Purified His-MyoGEF-501–790 was incubated with the in vitro-translated Myc-DH or Myc-PH and then subjected to coimmunoprecipitation assays with an antibody specific for Myc, followed by immunoblotting with antibodies specific for MyoGEF and Myc to detect His-MyoGEF-501–790 (top panel) and Myc-DH/Myc-PH (bottom panel), respectively. IP, immunoprecipitation; WB, Western blot; IgG-HC, immunoglobulin heavy chain; IgG-LC, immunoglobulin light chain.