FIGURE 9.

Effect of the T544D phosphomimetic mutation on actin filament formation. MDA-MB-231 cells transfected with a plasmid encoding either a wild-type MyoGEF carboxyl-terminal fragment (Myc-501–790-WT, a–c) or a phosphomimetic MyoGEF carboxyl-terminal fragment (Myc-MyoGEF-501–790-T544D, d–f) were subjected to immunofluorescence staining for actin filaments (red) and Myc-tagged polypeptides (green). Arrows indicate cells expressing Myc-MyoGEF-501–790-WT. Arrowheads indicate cells expressing Myc-MyoGEF-501–790-T544D. Scale bar = 20 μm.