Skip to main content
. 2014 Oct 15;289(49):34065–34073. doi: 10.1074/jbc.M114.604041

FIGURE 1.

FIGURE 1.

CRABP2 and HuR regulate a common subset of genes. A, MCF-7 cells were transduced with lentiviral particles harboring a non-targeting shRNA (shCtrl) or shRNA targeting CRABP2 (shCRABP2). Cells were selected with puromycin to generate cell lines stably expressing the respective shRNAs. Immunoblots demonstrate reduced expression of CRABP2. B, cells were cultured in delipidated medium for 48 h, treated with vehicle or RA (1 μm) for 4 days, and counted. Data are mean ± S.E. (n = 3). C and D, MCF-7 cells were transduced with lentiviral particles containing shRNAs targeting luciferase (shCtrl), CRABP2 (shCRABP2; C), or HuR (shHuR; D). Levels of mRNA for CRABP2 (C) or HuR (D) were assayed by qPCR. Data are mean ± S.E. (n = 3). *, p ≤ 0.01 by two-tailed Student's t test. E, Venn diagram depicting changes in gene expression in cells with reduced expression of CRABP2 and HuR. 135 genes were found to be regulated by both CRABP2 and HuR. F and G, expression profiles of genes commonly regulated by CRABP2 and HuR represented in a heat map clustering (F) or plotted as log2(-fold change) of genes regulated by CRABP2 versus HuR (G). Error bars represent S.E.