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IpaH9.8(244–545) exhibits hyperbolic kinetics. Shown is the concentration dependence of initial velocity versus [E2]o for Ubc5A (closed circles), Ubc5B (open circles), and Ubc5C (closed squares) in the presence of 25 pm IpaH9.8(244–545) in assays otherwise identical to those of Fig. 3. Lines, theoretical nonlinear hyperbolic regression fits of the data for kinetic constants summarized in Table 2. Inset, double reciprocal plot of the data of Ubc5B.
