TABLE 2.
Oligonucleotides used in this study
Restriction sites are underlined.
| Oligonucleotide | Sequence (5′–3′) | Comments |
|---|---|---|
| oCR149 | GCTGTTGACAATTAATCATCGG | pMMB207 fo (sequencing) |
| oCR150 | CGTTCTGATTTAATCTGTATCAGGC | pMMB207 re (sequencing) |
| oCR161 | AAAAACGCGGATCCATGAGCTTTAAAGGATTTAAAGTG | 5′ of lppA, BamHI |
| oCR162 | AAAAACGCTCTAGACTATTACTAGATATTGAGCTTTTTCCATTC | 3′ of lppA, XbaI |
| oAK7fo | GGACGCTCATGAAAAAAAATCACCACCATCATCACCACCTAGTTCCGCGTGGATCCGAATGG | BspHI/BamHI |
| oAK7re | CCATTCGGATCCACGCGGAACTAGGTGGTGATGATGGTGGTGATTTTTTTTCATGAGCGTCC | BamHI/BspHI |
| oKan3′ | TCACTTTCTGGCTGGATGATGG | 5′ out of KanR (deletion) |
| oKan5′ | GAATATGGCTCATAACACCCC | 3′ out of KanR (deletion) |
| oRM1 | AAAAACGCGGATCCCATCTAGTGCCATTTGCAAG | 5′ of lppA downstream region (deletion), BamHI |
| oRM2 | TTAGCGATACCTGGAAATAAG | 3′ of lppA downstream region (deletion) |
| oRM3 | GGTCATAACGATGCAAATAGTAG | 5′ of lppA upstream region (deletion) |
| oRM4 | AAAAACGCGGATCCAATAAATCCTTTTTAAATCCCTAAAGATG | 3′ of lppA upstream region (deletion), BamHI |
| oRM5 | AAAAACGCGGATCCATGAGCTTTAAAGGATTTAAAG | 5′ of lppA, BamHI |
| oRM6 | AAAAACGCGTCGACCTAGATATTGAGCTTTTTCCATTC | 3′ of lppA, SalI |
| oRM7 | GGCTGTCTGATTTTCCTCAAG | 3′ of lppA downstream region |
| oRM8 | CTGCCCGAATGGTATCAG | 5′ of lppA upstream region |
| oRM13 | AAAAACGCGGATCCCAAAGTTATGCCTCTAAATTG | 5′ of lppAΔ1–16, BamHI |
| oSE7 | GGTATCATGTCCACGCCCGTGGCGGGAAAGGC | 5′ of lppA C231A |
| oSE8 | GCCTTTCCCGCCACGGGCGTGGACATGATACC | 3′ of lppA C231A |
| oSE9 | GCCGTGGCGGGGCAGGCCGTACGACG | 5′ of lppA K235A |
| oSE10 | CGTCGTACGGCCTGCCCCGCCACGGC | 3′ of lppA K235A |
| oSE11 | CGTGGCGGGAAAGGCGCTACGACGACTGTTTTTGC | 5′ of lppA R237A |
| oSE12 | GCAAAAACAGTCGTCGTAGCGCCTTTCCCGCCACG | 3′ of lppA R237A |
| oSE13 | CCGTGGCGGGAAAGACCGTACGACGACTG | 5′ of lppA G236D |
| oSE14 | CAGTCGTCGTACGGTCTTTCCCGCCACGG | 3′ of lppA G236D |
| oWS25 | AGTTATGGATCCAAATTGGCATCCTCC | 5′ of lppA21–314, BamHI (crystallization) |
| oWS26 | CTAGATGTCGACTTATTACCATTCTGACCA | 3′ of lppA21–314, SalI (crystallization) |
| oWS73 | AAAAAAGGATCCGCCAAGGCGCCGGAGCAGACGGTGACGG | 5′ of phyA, BamHI |
| oWS91 | AAAAAAGTCGACCTACTTCGCCGGATGGCTCTTGAGCCAG | 3′ of phyA, SalI |