FIGURE 7.

Recombinant eNAMPT induces EMT in mammary epithelial cells. A and B, MCF10A cells were incubated for 14 days with or without (control) 100 ng/ml recombinant human eNAMPT. Thereafter, cells were imaged by light microscopy (A) and, finally, used either for RNA extraction and E-cadherin, N-cadherin, vimentin, and ZEB1 mRNA quantification by QPCR (B) or for confocal microscopy (C, E-cadherin detection, Qnuclear deep red (Qnuclear) nuclei staining, and their overlaps). D, HMLE cells were incubated for 14 days in 6-well plates with or without (control) 100 ng/ml recombinant human eNAMPT. Thereafter, RNA was extracted, and E-cadherin, N-cadherin, vimentin, and ZEB1 mRNA levels were quantified by QPCR. *, p < 0.05; **, p < 0.01. A–D, one representative experiment of three is presented.