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[3H]MPP+ uptake into proteoliposomes. [3H]MPP+ uptake was assayed as described in the legend for Fig. 2, with liposomes prepared from wild-type (■) and D25N-expressing (♦), E229Q-expressing (●), and E222Q/E229Q-expressing (▴) cells. Inset, for comparison, the results not corrected for protein content are shown for the wild type (■), E222Q/E229Q (▴), and mock liposomes (★).
