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. 2014 Oct 20;289(49):34250–34257. doi: 10.1074/jbc.M114.571182

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — IRF8 interacts with the BCL6 binding domain of BCOR. A, diagram of ANK domain- and BBD-deleted BCOR constructs. ANK, gray box; BBD, black box. B, HEK293 cells expressing V5-tagged ANK domain- or BBD-deleted BCORs and full-length IRF8 were lysed, pulled down with anti-V5 antibody, and subsequently blotted for V5 and IRF8. The BCL6 binding domain-deleted BCOR failed to co-immunoprecipitate with IRF8. The arrow indicates IRF8 protein. IgH, immunoglobulin heavy chain; IP, immunoprecipitation.