IRF8 interacts with the BCL6 binding domain of BCOR.
A, diagram of ANK domain- and BBD-deleted BCOR constructs. ANK, gray box; BBD, black box. B, HEK293 cells expressing V5-tagged ANK domain- or BBD-deleted BCORs and full-length IRF8 were lysed, pulled down with anti-V5 antibody, and subsequently blotted for V5 and IRF8. The BCL6 binding domain-deleted BCOR failed to co-immunoprecipitate with IRF8. The arrow indicates IRF8 protein. IgH, immunoglobulin heavy chain; IP, immunoprecipitation.