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. 2014 Oct 20;289(49):34250–34257. doi: 10.1074/jbc.M114.571182

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Synergistic repression of BCL6 activity by BCOR and IRF8. A, HEK293 cells containing each expression plasmid (BCL6, BCOR, and IRF8) and BCL6-responsive luciferase were lysed, and a luciferase assay was performed. Normalized luciferase (Luc) activity is the ratio of firefly luciferase activity to the expressed Renilla luciferase activity. Data shown are means ± S.E. (error bars). Student's t test was performed to evaluate statistical significance. *, p < 0.05. B, HEK293 cells expressing IRF8 and FLAG-tagged BLC6 were lysed, pulled down with anti-FLAG antibody, and subsequently blotted for FLAG and IRF8. The arrow indicates FLAG-BCL6 protein. IRF8 co-immunoprecipitated with FLAG-tagged BCL6. C, 18-81 and MPC11 cell lysates were precipitated with anti-IRF8 antibody and blotted for BCL6 and IRF8. Endogenous BCL6 was co-immunoprecipitated with IRF8 only in 18-81 cells. Immunoglobulin heavy chain (IgH) is shown as a loading control. The arrowhead indicates IRF8 protein. g, goat; r, rabbit; IP, immunoprecipitation.