FIGURE 2.

Transport activity and the effect of CuPh on inactivation. WT and the indicated mutants were transiently expressed in HeLa cells as described under “Experimental Procedures.” A, sodium-dependent [³H]GABA transport was measured at room temperature for 10 min. B, cells were incubated in the NaCl-containing medium with or without 30 μm CuPh for 5 min at room temperature, prior to the transport measurement. For A, the data are given as percentage of the activity of WT. Results represent mean ± S.E. (error bars) of at least three different experiments performed in quadruplicate. For B, the data are expressed as a percentage of untreated control and represent mean ± S.E. *, p < 0.05.