FIGURE 5.
Effect of EL2 mutations on the sensitivity of S359C mutants to CuPh. HeLa cells expressing the indicated S359C (A) or S359C/K448A (B) mutants were preincubated in NaCl-containing medium in the presence or absence of 30 μm CuPh for 5 min at room temperature. Subsequently, the cells were washed and assayed for [3H]GABA transport as described under “Experimental Procedures.” The data are given as a percentage of control. The transport activity of the non-treated mutants (percentage of WT) was 3.8 ± 1.1% (n = 8), 5.5 ± 1.6% (n = 9), 9.3 ± 1.7% (n = 10), 9.6 ± 1.6% (n = 8), 7.3 ± 2.0% (n = 9), and 12.2 ± 2.2% (n = 9) for S359C/C164S, S359C/C173S, S359C/C164S/C173S, S359C/K448A/C164S, S359C/K448A/C173S, and S359C/K448A/C164S/C173S, respectively (*, p < 0.05). Error bars, S.E.