FIGURE 9.

Effect of the composition of the external medium on the inhibition of EL2 mutants by MTSET. HeLa cells expressing C164S (A), C173S (B), or C164S/C173S (C) were preincubated in the presence or absence of 1 mm MTSET in media containing NaCl, NaCl + 1 mm GABA, choline chloride (ChCl), or sodium gluconate, as indicated. Values are given as a percentage of control (preincubation without MTSET in each condition) and represent the mean ± S.E. (error bars) of at least three different experiments done in quadruplicate. The transport activity of the non-treated mutants (percentage of WT) was 19.1 ± 2.9, 18.9 ± 3.4, and 31.8 ± 4.4% (n = 3) for C164S, C173S, and C164S/C173S, respectively, p < 0.05.