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Purification scheme for recombinant KLK2. Purification comprised negative IEC, activation of pro-KLK2 by EK, immobilized metal ion affinity chromatography (IMAC), BENAC, and size exclusion chromatography (SEC). Success of the individual steps was routinely monitored by SDS-PAGE. +DTT and −DTT indicates whether gels were run under reducing or oxidizing conditions, respectively. M, marker; L, load; F, flow-through; W, wash; E, elution; AU, absorbance units.
