FIGURE 5.
A, CIMO inhibits cell migration. 70 μl of HepG2 cells (5 × 105/ml) were seeded into each compartment of the culture insert, and the insert was removed after 12 h. The width of the wound was measured initially, and cells were incubated with and without CIMO (5 μm, 8 h) and CXCL12 (100 ng/ml, 24 h). B, CIMO inhibits the cell invasion. HepG2 (2 × 105) cells were seeded in the top chamber of BD BioCoatTM MatrigelTM. After preincubation with or without 5 μmol/liter CIMO for 8 h, transwell chambers were placed into the wells of a 24-well plate that contained either only basal medium or basal medium with CXCL12 (100 ng/ml) for 24 h. After incubation, the chamber was assessed for cell invasion by staining with crystal violet. Error bars, S.E.