ECP interactions with plant polysaccharides.
A, ELISA of spinach polysaccharide extracts (CDTA and NaOH) from roots and leaves with LM6 (pectin) and LM15 (hemicellulose) antibodies. B, ELISA of spinach polysaccharide extracts (CDTA and NaOH) or long arabinan chains (branched and unbranched) with E. coli JT1 expressing ECPSakai (pYR006), ECPIHE3034 (pMat3), or the control plasmid (pSE380). Significance levels are as follows: **, p ≤ 0.005; *, p ≤ 0.05; NS, non-significant. C, inhibition of antibody binding to spinach leaf pectin with E. coli JT1 expressing ECP. “% of inhibition” represents the percentage of antibodies bound compared with the control value (i.e. no bacteria). Bacteria were added at an A600 of 0.8. Values are the average ± S.D. of three experiments. Error bars represent S.D.