TABLE 1.

Primer sequences used in this study

Product	Primer name	Primer sequencea
ecpA–D	ecpAHindIII	CCCAAGCTTGTCCTCAATTCAACTCGG
	ecpDHindIII	CCCAAGCTTGCCGACCGAAATCGCCTG
ecpA–E	EcpAXbaI	GGACATCACGTCCTCTAGACAACTCGGG
	EcpEHindIII	TCTGAGGTGGAAAGCTTCCCTCGA
ecpR promoter	0324.5.XbaI	GGTCTAGACTTTAATTATGACTC
	0324.3.XbaI	GGTCTAGATACTTTCCAAACCTG
5′-flanking region of ecpA for deletions	0323.SalI.5F	GGAGAGTCGACGAAAAGATTTCGTGTTTATC
	0323.NotI.5R	CCGTTCCAAGCGGCCGCAAGAGCGTGTATTTCTTCCCGAGTTGAATTG
3′-flanking region of ecpA	0323.NotI.3F	CGCTCTTGCGGCCGCTTGGAACGGTCTCTCTGATGTACCAGCAGGG
deletions	0323.PstI.3R	GGTGCCTGCAGGGTACTGAAAGTGGTAGTTTG
3′-flanking region of ecpD for deletions	0320.Not1.3F	CGCTCTTGCGGCCGCTTGGAACGG TAAGGCCCTGCTGACAGCGGTCTG
	0320.PstI.3R	TTAATCTGCAGCTTAAACCACGTGTTGCCGGTG
ΔecpA.NotI linker	0323.SalI.5F	For splice overlap extension PCR of products ecpA.5.Not and ecpA.3.Not PCR products
	0323.PstI.3R
ΔecpA–D.NotI linker	0323.SalI.5F	For splice overlap extension PCR of products ecpA.5.Not and ecpD.3.Not PCR products
	0320.PstI.3R

^a Restriction enzyme sequences are underlined.