FIGURE 6.

Roles of SelH in intracellular GSH content and apoptotic death in the response to H₂O₂ treatment in HeLa cells. A, early passage cells were treated with H₂O₂ (0–160 μm) for 24 h, followed by staining of trypsinized single cells with mBCL for flow cytometric analysis. B, cells were treated with H₂O₂ (160 μm) and harvested for Mitocapture staining to assess apoptosis by determining the disruption of mitochondrial membrane potential. Representative pictures were shown (green, apoptotic cells; red, live cells). The percent apoptotic cells were presented with mean ± S.E. (n = 8). C, clonogenic assay was performed after treatment with H₂O₂ alone or in combination with NAC. The number of colonies in SelH and scrambled shRNA cells without H₂O₂ or NAC treatment was set as 100%. Values are means ± S.E. (n = 3). *, p < 0.05, compared with scrambled shRNA cells.