FIGURE 7.

Roles of SelH in the nuclear accumulation of phosphorylated Nrf2 after H₂O₂ treatment. A, nuclear fractions from SelH shRNA and scrambled shRNA HeLa cells treated with H₂O₂ for 24 h were applied for Western analysis. B, immunoblotting intensity was quantified by ImageJ and nuclear pNrf2 expression was normalized by lamin B and presented with means ± S.E. (n = 3). C, SelH shRNA and scrambled shRNA MRC-5 cells were seeded onto coverslip, treated with H₂O₂ (20 μm), and recovered during the course of 5 days in fresh medium. Fluorescence intensity of nuclear pNrf2 was measured, set as 100% in scrambled shRNA cells without H₂O₂ treatment, and presented with means ± S.E. (n = 6). *, p < 0.05, compared with scrambled shRNA cells.