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. 2014 Oct 23;289(49):34408–34421. doi: 10.1074/jbc.M114.615393

FIGURE 5.

FIGURE 5.

PFB0075c-PFB0100c cluster and clag9 are intact in PFB0080c knock-out P. falciparum. A, a 100-bp fragment corresponding to the deleted region of PFB0080c was PCR-amplified from the genomic DNA isolated from the parental C4S-adherent and ΔPFB0080c parasites selected under WR99210 and 5-fluorocytosine (FC) pressure. Although the PFB0080c fragment is present in parental C4S-adherent parasites, it is completely absent in ΔPFB0080c parasites selected under the pressure of both WR99210 and 5-fluorocytosine. B, fragments corresponding to the coding regions of the indicated genes (clag9 present in chromosome 9 and genes of the PFB0075c-PFB0100c cluster in chromosome 2) that are located in the subtelomeric regions were amplified from the genomic DNA of ΔPFB0080c parasites using the primers listed in Table 1. Seryl tRNA was analyzed as a control. All genes except for PFB0080c were amplified, indicating that subtelomeric regions of chromosomes 2 and 9 that contribute to cytoadherence are intact in ΔPFB0080c parasites.