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. 2014 Oct 23;289(49):34408–34421. doi: 10.1074/jbc.M114.615393

FIGURE 6.

FIGURE 6.

PFB0080c-knock-out P. falciparum express VAR2CSA on the surface of infected erythrocytes. A and B, immunofluorescence analysis of VAR2CSA in live ΔPFB0080c IRBCs was performed using rabbit anti-VAR2CSA antiserum followed by biotin-conjugated sheep anti-rabbit IgG secondary antibody and phycoerythrin-conjugated streptavidin. Shown are the immunofluorescent and light micrographs of parental 3D7-CSA parasites (A) and ΔPFB0080c parasites (B); both parasite types expressed VAR2CSA protein. DIC, differential interference contrast.