FIGURE 8.

PFB0080c knock-out P. falciparum express genes of PFB0075c-PFB00100c cluster. RNA preparations from 3D7-CSA and ΔPFB0080c parasites were reverse-transcribed using SuperScript II reverse transcriptase, and the cDNA thus obtained was analyzed for the indicated genes by quantitative PCR using SYBR Green PCR mix and nucleotide primers listed in Table 1. The analysis was performed in triplicate. The relative levels of gene expression were normalized to fructose bisphosphate aldolase. Data are expressed as mean values ± S.E. (error bars). *, p < 0.05; **, p < 0.01.