FIGURE 9.

PFB0080c binds to VAR2CSA in a dose-dependent manner. ELISA analysis was performed to assess the binding of the purified GST-tagged recombinant PFB0080c to the His-tagged recombinant full-length extracellular portion of VAR2CSA. Ninety-six-well microtiter plates were coated with 5 μg/ml PFB0080c and control proteins GST and BSA. After blocking the wells, VAR2CSA at the indicated concentrations was added to each well. The bound VAR2CSA was detected using mouse anti-His₅ antibody and HRP-conjugated goat anti-mouse secondary antibody as described under “Experimental Procedures.” The analysis was performed three times, each in duplicate. The data are expressed as mean values ± S.E. (error bars). *, p < 0.01.