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. 2014 Dec 4;9(12):e112454. doi: 10.1371/journal.pone.0112454

Figure 4. Real-time PCR analysis of 14 day murine IVD explants compared to freshly harvested IVD explants.

Figure 4

Genes related to structural extracellular matrix production (a) and extracellular matrix remodeling genes (b) were analyzed. After 14 days in culture, murine IVD explants do not change the gene expression of the principal structural genes of the IVD, while ADAMTS4 and TIMP1 are significantly upregulated. Analysis was normalized to GAPDH. Results are presented relative to freshly harvested murine IVDs where the expression value was set to 1. * denotes statistical significance (p<0.05).