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. 2014 Dec 4;9(12):e114126. doi: 10.1371/journal.pone.0114126

Figure 6. Gap enriched puncta are apparent in the myotube during overt fusion.

Figure 6

(A) Time lapse of stage 13 wild-type embryo expressing twi>gap-GFP, sns-nls-mCherry, and twi>Actin-mCherry. The mCherry signals were collected in the same channel but note that sns-nls-cherry marks the nuclei and twi>Actin-mCherry was very weak and is not clearly visible under these conditions. See Fig. S2 and Movie S6 for entire fusion event. As the FCM nucleus is absorbed into the myotube, gap-GFP containing puncta are apparent near the site of fusion. (B) Time lapse of stage 13 wild-type embryo expressing twi>gap-mCherry and twi>Actin-GFP. See Movie S7 for entire fusion event. Gap-mCherry containing membrane remnants in the myotube are visible near sites of fusion. Arrows mark the membrane-associated puncta. Scale bar: 5 µm.