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. 2014 Dec 4;9(12):e114516. doi: 10.1371/journal.pone.0114516

Figure 7. Regulation of the IL-1-induced activity of the human IL23A promoter by IκB-ζ.

Figure 7

(A) Schematic representation of the human IL23A gene and strategy for the preparation of pGL3 luciferase reporter vectors under the control of different lengths of wild type or mutated IL23A 5′ upstream promoter sequences. The major predicted binding sites for TFs are depicted. (B) HeLa cells were transfected with a basic pcDNA3.1 plasmid, pcDNA(Basic), or a pcDNA(NFKBIZ) along with pGL3 luciferase reporter vectors under the control of different lengths of wild type or NF-κB-mutant promoter of IL23A. At 24 h after transfection, cells were treated with IL-1β. Luciferase activity was read in cell lysates after 48 h of stimulation. Data were normalized to TK-Renilla activity and expressed relative to the normalized activity of the Basic vector or as mean fold change ± SEM in normalized luciferase activity induced by the pcDNA(NFKBIZ) with respect to the control vector pcDNA(Basic) to highlight the NFKBIZ-stimulated luciferase activity from each reporter vector shown. Results are mean ± SEM, n = 6.