Fig. 10.

Unique molecular behaviors of M5-HMM visualized by HS-AFM. a Successive HS-AFM images showing a foot stomp event that occurred at the leading-head (ATP, 1 μM; frame rate, 7 fps; scan area, 150 × 75 nm² with 80 × 40 pixels; scale bar 50 nm). The leading-head detachment is marked with the light-blue arrowheads. The corresponding AFM movie can be seen at the following website: http://www.nature.com/nature/journal/v468/n7320/extref/nature09450-s3.mov b HS-AFM images before (upper panel) and after (lower panel) unwinding of coiled-coil tail observed in 50 μM ADP. The corresponding AFM movie can be seen at the following website: http://www.nature.com/nature/journal/v468/n7320/extref/nature09450-s5.mov c HS-AFM image showing the leading head with a sharply bent conformation observed in the nucleotide-free condition. The corresponding AFM movie can be seen at the following website: http://www.nature.com/nature/journal/v468/n7320/extref/nature09450-s6.mov d HS-AFM image showing nucleotide-free M5-HMM with heads bound to adjacent actin subunits (upper panel) and its illustration (lower panel). Imaging conditions for (b), (c) and (d) are as followings: frame rate, 3 fps; scan area, 100 × 100 nm² with 80 × 80 pixels; scale bar 30 nm. The vertical dashed lines represent the centers of mass of the motor domains, and the plus signs indicate the plus ends of actin