Fig. 4.

Schematics showing in vitro motility assay systems for actomyosin. a Myosin-coated bead assay. The myosin-coated fluorescent beads are subjected to the polar arrays of actin cables naturally formed on chloroplastd of the alga Nitella, and movement of the beads are observed under a florescent microscope. b Actin filament gliding assay. Myosin molecules are attached to the surface of a nitrocellulose-coated coverslip and gliding motion of the fluorescently labeled actin filaments are observed under a fluorescence microscope