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. 2014 Dec 4;10(12):e1004547. doi: 10.1371/journal.ppat.1004547

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© 2014 Embley et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Rat antibody to the mitosomal marker Hsp70 (a, e and i) labelled discrete structures (mitosomes) inside the parasites (green) whereas rabbit antisera to ThNTT1 (b), ThNTT3 (f) and ThNTT4 (j) labelled the surface of the parasite (red). The nuclei of rabbit kidney host cells (large nuclei) and parasites (small nuclei) were labelled with DAPI (blue). c, g and k; DIC images with the three merge channels. d, h and l, enlarged images of individual clusters of T. hominis showing antibody labelling. White arrow shows aggregation of spores within the host cell. Yellow arrows show the meront or vegetative stage of the parasite.