Figure 3. Blocking JAM-C increases the number of DCs migrating to the draining lymph node.

(A) The ear draining lymph node cells were harvested and stained for FACS analysis 18 hours after FITC-painting. Representative FACS dot plots are shown. (B) The number of IA^high CD11c⁺ FITC⁺ migratory DCs was counted. Data are expressed as a percentage of the mean of the control group ± SEM of eighteen mice per group pooled from 3 separate experiments, and were analyzed by the unpaired Student's t test with ***: p<0.001. Raw data from one representative experiment are provided in S7 Figure.