Figure 4. Shifting V. cholerae from glucose-based to starch-based conditions leads to a decrease in CT production.

Bacterial cultures were induced for virulence in the presence of glucose for 30 min, washed (where indicated) and shifted to virulence-non-inducing or virulence-inducing medium containing either glucose or starch. The amount of CT was determined as described for Figure 2. Statistically significant differences were determined through Student's t-test (* P≤0.05).