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HP1BP3 depletion inhibits cancer cell renewal during hypoxia/re-oxygenation. HP1BP3-depleted A431 cells were cultured in 48 h normoxia (Nx), 48 h hypoxia (Hx), or 24 h hypoxia/24 h re-oxygenation (Rx) and then assessed for tumor sphere formation over 10 days of culture (A and B). C, size distribution of tumor spheres formed by HP1BP3-depleted A431 cells subjected to the same conditions. Each experiment was performed in triplicate (*p < 0.05, **p < 0.005).
