Fig. 10.

Western blot quantification of selected proteins evident as having been regulated in hRSV-infected A549 cells using both in-solution protein IEF and protein fractionation-free workflows. Equal quantities of protein from lysates of mock- or hRSV-infected A549 cells were analyzed by Western blotting for the indicated proteins. All proteins analyzed were found to be regulated within a FDR cutoff of less than 1% by all label-free mass spectrometry workflows. Four independent sets of infected samples were analyzed in triplicate. Integrated intensities are presented as the mean ± S.E. (n = 4). Statistical significance was determined using a one-way ANOVA with Dunnetts correction. † and § denote a statistically significant difference from mock at p < 0.01 and p < 0.0001, respectively. Raw Western blots are presented for one biological replicate in supplemental Fig. S7A as an overall representation of the data used in this quantitative analysis.