Fig. 4.

Relative abundances of viral proteins in hRSV infected cells estimated by the top3 method: A, top3 calculations for each hRSV protein detected in infected A549 cells for the four replicates analyzed by the protein fractionation-free workflow with each top3 value expressed as a proportion of the total top3 values for all proteins calculated for the respective replicates; B, the order of relative abundances apparent from the plot above with the average numbers of tryptic peptides unique to each identified protein in the four analyses performed presented in parentheses; and, C, the coding order of the hRSV genome from which the genes are transcribed with a polarity of decreasing efficiency from 3′ to 5′ termini, implying a corresponding polarity in protein abundances.