Fig. 5.

Protein groups evident as being regulated by analysis of in-solution protein IEF workflow datasets: A, comparison of spectral count (yellow circles) and intensity (blue circles) based quantification for identifying overall regulation observed using a FDR cut-off of less than 1%; MA plots showing significantly regulated protein groups in the B, spectral count and C, intensity data as black dots; and, distribution of protein groups observed to be D, up-regulated and E, down-regulated in hRSV- compared with mock-infected A549 cells by spectral counts (yellow circle) and intensity (blue circle) measurements with the overlapping intersections representing protein groups evident as significantly different by both methods.