Fig. 5.
Non-covalent peptide binding by Ost6p in vitro. A, aligned AspN peptides from MBP-Gas1 and MBP-Gas1R90Q detected via LC-ESI-MS/MS. Extracted ion chromatograms of peptide from MBP-Gas1 (solid) and MBP-Gas1R90Q (dashed) in (B) load, and second wash fraction with (C) reduced MBP-Ost6 and (D) oxidized MBP-Ost6. E, relative enrichment of the Gas177–108 versus the Gas1R90Q 77–108 peptide by columns with attached oxidized MBP-Ost6. Data are average of triplicates. Error bars indicate range.